In future chemistry requires enzymes which are robust, stable and highly efficient. This can be achieved through enzyme engineering that requires modification of enzymes active and/or binding site using site-directed mutagenesis or site saturated mutagenesis. The new enzymes will not only broaden the substrate scope but will only be highly stereo-, regio- and chemoselective.
Combing the use of enzymes with chemical/non-enzymatic methods can be a very useful tool for the synthesis of complex natural products as demonstrated by us for bisanthraquinones and dihydroarenediols synthesis.
Natural products isolated from fungus and bacteria have a complex biosynthetic pathway that involves many enzymes for their production. Elucidation of their biosynthesis can be very useful devising methodologies for the natural products which are short and more environmentally friendly. Understanding of biosynthetic pathways could help us produce natural products which are scarcely available from natural resources and are difficult to synthesize chemically.
The enzymes of our interest are
i) C-C bond forming enzymes (Cytochrome c)
ii) Naphthol redcutase enzymes (NADPH dependent Tetrahydroxynaphthalene reductase, trihydroxynaphthalene redcutase etc.)
iii) Oxidoreductases (NADPH dependent keto reductases etc)
In our group, we are working on to investigate the role of superoxide in modifying biomolecules such as DNA, proteins and lipids. This could answer many questions pertaining to the role of oxidative stress in causing diseases as well as in gaining the molecular level information.